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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and plays a essential part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually explained. Two additional splice variants ended up documented but comprehensive nucleotide sequence information and facts just isn't however available.
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Blocking peptides are peptides that bind specially towards the goal antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.
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This information outlines additional feasible optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This guideline outlines further possible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, you can see click here which staining is distinct.
Target Data Cyclin E belongs into the really conserved cyclin spouse and children, whose members are characterised by a spectacular periodicity in protein abundance in the cell cycle. Cyclins function as regulators of CDK kinases. Various cyclins show unique expression and degradation designs which contribute to the temporal coordination of every mitotic function. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates with the G1-S period boundary which is degraded as cells development as a result of S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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